NanoTYPE Product Family Basic Operations Training

Oxford Nanopore Technologies Overview

NanoTYPE Product Overview

Technical Product Overview – Assay - General Assay Workflow

Laboratory Session:

·        Long-Range PCR Setup

·        Flow Cell Priming Practice

·        Sample Quantitation & Normalization

·        Library Preparation (sample barcoding, pooling, and cleaning)

·        Flow Cell Priming

·        Sequencer Setup

·        Library Loading and Sequencer Start

·        MinKNOW Sequencing Software / Key Metrics

·        NanoTYPE MONO Long-Range PCR Setup

Q/A Session, Daily Wrap-up

Previous Day Review -Daily Agenda

HLA-11 Plus Assay Overview

Laboratory Session:

·        HLA-11 Plus long -range PCR Practice

·        Flow Cell Washing/Storage Procedures

·        MONO Library Preparation (Quantification and Normalization sample barcoding, pooling, and cleaning)

·        Sequencer Setup

·        Post-Run Data Collection

·        Flow Cell Washing MONO Data Collection  

·        Files to Collect - How/Where to Import Files

·        HLA-11 Plus Normalization HLA-11 Plus Library Preparation Sequencer Setup  

·        MinKNOW Software Post-Run Data Collection

Q/A Session- Daily Wrap-up

Summarize Previous Day - Review Daily Agenda

Wet Lab – Flow Cell Washing/Storage Procedures

NanoTYPE Software Overview

·        Genotyping Algorithm and Software Theory

·        NanoTYPE MONO Data Collection

·        NanoTYPE Sample Analysis Workflow

·        NanoTYPER Sample Analysis Examples

·        Participant Sample Analysis

Q&A Session - Daily Wrap-up