FLEXTYPE and Geney Basic Operations Training

Agenda

Day 1

Introductions  

Technical Product Overview Recap - Following Virtual Training  

Laboratory Session:  

·        Amplification and Illumina Assay Workflow   

·        PCR Setup   

·        Post-Amp Preparation: Amplicon Purification, Normalization, Fragmentation  

·        Library Preparation:  Adaptor Ligation  

·        ONT flow cell practice  

·        Library Preparation: Pooling, Size Selection, Library Amplification, Bead Clean  

·        Library Quantification, Dilutions, Denaturation, Sequencer Loading  

Q&A Session  Daily Wrap-up 

Day 2

Review Daily Agenda 

Laboratory Session:  

·        ONT Assay Workflow  

·        Post-Amp Preparation: Amplicon Purification, Normalization  

·        Library Preparation: Barcoding, Pooling, Bead Clean  

·        Adaptor Attachment, Flow Cell Priming, Sequencer Loading  

·        Software Sesson:  

·        Geney Software Navigation Geney Sample Analysis Examples  

·        Participant Sample Analysis Flow Cell Storage  

Q&A Session Daily Wrap-up

Day 3

Review Daily Agenda  

Independent ONT Protocol Practice  (bench work only, no cycling or sequencing)  

Independent Illumina Protocol Practice  (bench work only, no cycling or sequencing)  

Available Resources 

Q&A Session  Daily Wrap-up 

Evaluations