LIFECODES Antibody and SSO HLA Typing Training

Day 1

Introductions to HLA (PPT)

LIFECODES SSO assay protocol overview (PPT)

Laboratory Session

• Trainer demonstration of LIFECODES SSO testing with samples
• Locus Specific Amplification PCR
• Luminex Instrument Calibration and batch sep up
• Probes Hybridization
• Data acquisition in the Luminex Instrument

Day 2

LIFECODES SSO test run by participants with samples

• Luminex Instrument Start up, Calibration and Verification
• Auto batch Set-Up in MATCHIT! Software
• Set-Up Batches in xPONENT Software
• Perform Locus B eRES, DRB1 eRES & DQA/DQB assay

LIFECODES MATCH IT! DNA software overview (PPT)

LIFECODES SSO Data analysis

• Import Template file into MatchIT! software
• Import CSV file into Match IT for analysis
• Review of Days 1 & 2 and Q&A

Day 3

Overview of LIFECODES Antibody workflow (PPT)

Assay protocol review (PPT)

Trainer Demonstration of LIFECODES Antibody testing with samples

• Perform LIFECODES Screening (LMX), ID (CI or CII), Single Antigen (LSA CI or CII) assay
• Demonstrate C3d assay

LIFECODES Antibody test run by participants with samples

• Perform LIFECODES Screening (LMX), ID (CI or CII), Single Antigen (LSA CI or CII) assay
• Luminex Instrument Start up, Calibration and Verification
• Auto batch Set-Up in MATCHIT! Software
• Set-Up Batches in xPONENT Software
• Perform Luminex Shutdown procedure

Day 4

Overview of MATCH IT! Antibody Software (PPT)

Sample Run Analysis with MATCH IT! Antibody Software

• Review software calculations and interpret results from LMX, ID, LSA, and C3d assays.
• Guided LIFECODES Antibody result analysis I

Day 5

Overall Review and Q&A

Course evaluation