LIFECODES Antibody and SSO HLA Typing Training

Agenda

Day 1

Introductions to HLA (PPT)

LIFECODES SSO assay protocol overview (PPT)

Laboratory Session

·        Trainer demonstration of LIFECODES SSO testing with samples

·        Locus Specific Amplification PCR

·        Luminex Instrument Calibration and batch sep up

·        Probes Hybridization 

·       Data acquisition in the Luminex Instrument

Day 2

LIFECODES SSO test run by participants with samples

·        Luminex Instrument Start up, Calibration and Verification

·        Auto batch Set-Up in MATCHIT! Software

·        Set-Up Batches in xPONENT Software

·        Perform Locus B eRES, DRB1 eRES & DQA/DQB assay

LIFECODES MATCH IT! DNA software overview (PPT)

LIFECODES SSO Data analysis

·        Import Template file into MatchIT! software

·        Import CSV file into Match IT for analysis 

·       Review of Days 1 & 2 and Q&A

Day 3

Overview of LIFECODES Antibody workflow (PPT)

Assay protocol review (PPT)

Trainer Demonstration of LIFECODES Antibody testing with samples

·        Perform LIFECODES Screening (LMX), ID (CI or CII), Single Antigen (LSA CI or CII) assay

·        Demonstrate C3d assay

LIFECODES Antibody test run by participants with samples

·        Perform LIFECODES Screening (LMX), ID (CI or CII), Single Antigen (LSA CI or CII) assay

·        Luminex Instrument Start up, Calibration and Verification

·        Auto batch Set-Up in MATCHIT! Software

·        Set-Up Batches in xPONENT Software 

·       Perform Luminex Shutdown procedure

Day 4

Overview of MATCH IT! Antibody Software (PPT)

Sample Run Analysis with MATCH IT! Antibody Software

·        Review software calculations and interpret results from LMX, ID, LSA, and C3d assays.

·        Guided LIFECODES Antibody result analysis I

Day 5

Overall Review and Q&A

Course evaluation