NanoTYPE Training

Day 1

Oxford Nanopore Technologies Overview

NanoTYPE Product Overview

Technical Product Overview – Assay - General Assay Workflow

Laboratory Session:

• Long-Range PCR Setup
• Flow Cell Priming Practice
• Sample Quantitation & Normalization
• Library Preparation (sample barcoding, pooling, and cleaning)
• Flow Cell Priming
• Sequencer Setup
• Library Loading and Sequencer Start
• MinKNOW Sequencing Software / Key Metrics
• NanoTYPE MONO Long-Range PCR Setup

Q/A Session, Daily Wrap-up

Day 2

Previous Day Review -Daily Agenda

Laboratory Session:

• Flow Cell Washing/Storage Procedures
• MONO Library Preparation
• Sequencer Setup
• Library Loading and Sequencer Start
• Post-Run Data Collection
• Files to Collect - How/Where to Import Files

NanoTYPE Software Overview

• Genotyping Algorithm and Software Theory
• NanoTYPE MONO Data Collection
• NanoTYPE Sample Analysis Workflow
• NanoTYPER Sample Analysis Examples
• Q/A Session- Daily Wrap-up

Day 3

Summarize Previous Day - Review Daily Agenda

Wet Lab – Flow Cell Washing/Storage Procedures

Participant Sample Analysis

Q&A Session - Daily Wrap-up